Journal: European Journal of Translational Myology

Article Title: Role of the ubiquitin-proteasome system in the sarcopenic-like phenotype induced by CCL5/RANTES

doi: 10.4081/ejtm.2024.12249

Figure Lengend Snippet: CCL5/RANTES increases atrogin-1 and MuRF-1 protein levels in C2C12 myotubes . C2C12 myoblasts were differentiated for 5 days. Then, myotubes were incubated with 200 ng/mL of recombinant CCL5/RANTES (rCCL5) or vehicle for 24 h. (A) MuRF-1 and (B) atrogin-1 protein levels were detected for western blot analysis using β‐actin as a loading control. Molecular weights are shown in kDa. Densitometry analysis for MuRF-1 (C) and atrogin-1 (D) is represented as a fold of change relative to the vehicle and expressed as the mean ± SD of three independent experiments. (t-test, * p < 0.05 vs. vehicle group).

Article Snippet: The immunoblotting was developed with the following primary antibodies: mouse anti-MHC (1:1,000 MF-20; Developmental Studies, Hybridoma Bank, University of Iowa, Iowa, IA, USA), mouse anti-troponin I (1:1,000; Cell Signaling, Danvers, MA, USA), mouse anti-MuRF-1 (1:1,000, Abcam, Cambridge, MA, USA), rabbit anti-atrogin-1 (1:1,000, Abcam, Cambridge, MA, USA) mouse anti-α-Tubulin (1:1,000; Santa Cruz, Dallas, TX, USA), mouse anti-β-actin (1:2,000, Abcam, Cambridge, MA, USA).

Techniques: Incubation, Recombinant, Western Blot